论文集锦 II--pco.flim:

2021-06-08 00:12:43 admin
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PCO 成像技术

pco.flim:
Luminescence Lifetime Imaging of Chemical Sensors


The pco.flim is the first luminescence decay time microscopy camera using a 2-tap CMOS image sensor. Synchronized modulation of pixels and stimulated light enables the analysis of decay times in the range of 100 ps to 100 µs. The pco.flim has a resolution of 1008 x 1008 pixels. It reads out up to 45 double frames/s.

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Using a C-mount optical interface, the pco.flim is easy to connect to any microscope. This allows its integration in versatile fields of applications.

The following paper describes one of them:

Luminescence Lifetime Imaging of Chemical Sensors - A Comparison between Time-Domain and Frequency-Domain Based Camera Systems
by Klaus Koren, Maria Moßhammer, Vincent V. Scholz, Sergey M. Borisov, Gerhard Holst and Michael Kühl, Analytical Chemistry 2019

Abstract
Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.

Download and read the full research paper:
www.pco.cn/applications/life-s

cience/llim-of-chemical-sensors

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